A Review of Fifteen Years Developing Computational Tools to Study Protein Aggregation

The presence of insoluble protein deposits in tissues and organs is a hallmark many human pathologies. In addition, the formation aggregates considered one main bottlenecks to producing protein-based therapeutics. Thus, there high interest rationalizing predicting aggregation. For almost two decades, our laboratory has been working provide solutions for these needs. We have traditionally combined core tenets both bioinformatics wet lab biophysics develop algorithms databases study aggregation its functional implications. Here, we review computational toolbox developed by lab, including programs identifying sequential or structural aggregation-prone regions at individual proteome levels, engineering solubility, finding evaluating prion-like domains, studying disorder-to-order transitions, categorizing non-conventional amyloid polar nature, among others. perspective, succession tools describe illustrates how understanding phenomenon evolved over last fifteen years.